Production and activity optimization of cellulase and xylanase from the compost isolated Paracoccus kondratievae strain GB in Egypt

Menshawy, Mahmoud N.; Abdel-Hamid, Ahmed M.; El-Katatny, Mo&#039;men; Arafat, Hussam H.

doi:10.21608/jmr.2023.218173.1112

Production and activity optimization of cellulase and xylanase from the compost isolated Paracoccus kondratievae strain GB in Egypt

Document Type : Original Article

Authors

¹ Department of Botany &amp; Microbiology, Faculty of Science, Minia University, Minia, 61519, Egypt

² Department of Botany &amp;amp; Microbiology, Faculty of Science, Minia University, Minia, 61519, Egypt

10.21608/jmr.2023.218173.1112

Abstract

The production and activity of both cellulase and xylanase from Paracoccus kondratievae strain GB with accession number (MW532124) isolated from compost were determined. The effect of various parameters on enzymes production including pH, temperature, carbon source, nitrogen source, incubation time, and agitation speed was investigated. Enzyme activity was also assessed by estimating the impact of temperature, pH, and incubation period. The highest value of cellulase production was accomplished at an incubation temperature of 35 °C (25.9 pKat ml-1), pH 8.0 (46.96 pKat ml-1), agitation speed of 150 rpm (47.8 pKat ml-1), incubation period of 5-7 days (41.8 pKat ml-1), CMC as a carbon source (205.8 pKat ml-1) and ammonium sulphate as a nitrogen source (200.15 pKat ml-1). While, xylanase was produced maximally at 35 °C (4.9 nKat ml-1), pH 8.0 (6.45 nKat ml-1), agitation speed of 150 rpm (4.15 nKat ml-1), incubation period of 4 days (4.15 nKat ml-1), xylan as a carbon source (4.5 nKat ml-1) and ammonium nitrate as a nitrogen source (6.66 nKat ml-1). On the other hand, the highest xylanase activity was obtained at 45 °C and pH 6.0 after 50 minutes of incubation, whereas, the highest cellulase activity was obtained at 45-55 °C and pH 6.0 after 60-70 minutes. These results show the thermophilic nature of these two examined enzymes. Our findings suggest that the thermophilic glycosyl hydrolases produced Paracoccus kondratievae strain GB (MW532124) might be good candidates for the degradation of lignocellulosic waste for industrial applications.

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