Effect of treatment Osteosarcoma (Mg-63) cells with Vitamin C, Green tea and their combination (An-in vitro study)

Document Type : Research Articles

Authors

1 Faculty of Dentistry, Minia University

2 Department of Oral Pathology, Faculty of Dentistry ,Minia University

3 Department of Oral Pathology ,Faculty of Dentistry , Minia University.

4 Department of Oral Pathology, Faculty of Dentistry , Minia University

Abstract

Abstract
Background: Osteosarcoma (OS) is the most common primary bone malignancy that affects children and adolescents. It is considered as a serious threat to the human health globally. For many years plants and natural constitutes components such as vitamin C (ascorbic acid), green tea have been used as anti-cancer drugs.
Aim: The objective of this in vitro study was to investigate the effect of vitamin C, green tea and their combination on Mg-63 cell line.
Material Methods: Treatment of cell line (Mg-63) by different concentrations of vitamin, green tea and their combination was done to evaluate the viability of the treated cells by SRB assay. Microscopic examination and were applied. Results: Regarding cytotoxicity effect of vitamin C, green tea and their combination on Mg-63cell line, it was noticed that cell distribution showed a variable arrest at different phase of cell division. Where there was non-significant difference of arrested cells of these drugs compared with its value in non-treated G0-G1 phase control cells while there was a significant elevated arrest of treated cells during the G2-M phase and the significant difference of cell arrest at G2-M phase was type of treatment related.
Conclusion: From the results of the present study, we noticed that vitamin green tea and their combination have cytotoxic effect on Mg-63 cell line, it also induced an effect on the cell cycle distribution, resulting in apoptosis, necrosis and autophagy.

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Journal of Modern Research
Volume 4, Issue 1
January 2022
Pages 27-31

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