Bahgat, S., Anwar, G., Mohamed, M., Ahmed, K. (2024). In vitro culture and cytological features of two Moringa species cultivated in Egypt. Journal of Modern Research, 6(2), 105-112. doi: 10.21608/jmr.2024.280789.1123
Shymaa Osama Bahgat; Gehan Mohamed Anwar; Mahmoud Abd El-hakem Mohamed; Kasem Zaki Ahmed. "In vitro culture and cytological features of two Moringa species cultivated in Egypt". Journal of Modern Research, 6, 2, 2024, 105-112. doi: 10.21608/jmr.2024.280789.1123
Bahgat, S., Anwar, G., Mohamed, M., Ahmed, K. (2024). 'In vitro culture and cytological features of two Moringa species cultivated in Egypt', Journal of Modern Research, 6(2), pp. 105-112. doi: 10.21608/jmr.2024.280789.1123
Bahgat, S., Anwar, G., Mohamed, M., Ahmed, K. In vitro culture and cytological features of two Moringa species cultivated in Egypt. Journal of Modern Research, 2024; 6(2): 105-112. doi: 10.21608/jmr.2024.280789.1123
In vitro culture and cytological features of two Moringa species cultivated in Egypt
1Genetics Department, faculty of Agriculture, El-minia university, El-Minia Egypt
2Department of genetics, Faculty of Agriculture, Minia University
3Horticulture Department, Faculty of Agriculture, Minia University, El-Minia, Egypt
4Genatic, Faculty of agricultre, Minia University
Abstract
Abstract The significance of Moringa lies in its diverse advantages, encompassing nutritional richness, medicinal properties, and potential environmental sustainability. The current study sheds light on the impact of explant type and medium composition on callus and shoot induction of two Moringa species cultivated in Egypt. Additionally, it investigates certain cytological features in root tip cells of those plants as well as cells of induced calli in vitro, such as cell shape, size and mitotic activity. The results revealed notable differences in callus induction and its fresh and dry weights between the two Moringa species (M. oleifera and M. stenopetala), as well as among MS media used. Furthermore, the study demonstrated significant variations in response of two tested explants (leaves and hypocotyls). Active meristematic cells were classified into three types: Type I includes dividing cells, Type II consists of meristematic cells with diffused chromatin, and Type III represents cylindrical cells with diffused chromatin. Interestingly, the percentages of dividing cells may suggest the mitotic index (MI), with the MI being considerably higher in cells of M. oleifera compared to M. stenopetala.
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